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Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase
Joint Authors
Dash, Biplab Kumar
Rahman, M. Mizanur
Sarker, Palash Kumar
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A study was carried out with a newly isolated bacterial strain yielding extracellular amylase.
The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp.
and identified as Bacillus subtilis BI19.
The effect of various fermentation conditions on amylase production through shake-flask culture was investigated.
Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly.
A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield.
Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0.
Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively.
Amylase production was 3.06 times higher when optimized production conditions were used.
Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively.
The crude enzyme showed activity and stability over a fair range of temperature and pH.
These results suggest that B.
subtilis BI19 could be exploited for production of amylase at relatively low cost and time.
American Psychological Association (APA)
Dash, Biplab Kumar& Rahman, M. Mizanur& Sarker, Palash Kumar. 2015. Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057040
Modern Language Association (MLA)
Dash, Biplab Kumar…[et al.]. Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1057040
American Medical Association (AMA)
Dash, Biplab Kumar& Rahman, M. Mizanur& Sarker, Palash Kumar. Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057040
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057040