Associations of LIG4 and HSPB1 Genetic Polymorphisms with Risk of Radiation-Induced Lung Injury in Lung Cancer Patients Treated with Radiotherapy

Abstract EN

Objective.

This study aims to explore the correlations of genetic polymorphisms in LIG4 and HSPB1 genes with the radiation-induced lung injury (RILI), especially radiation pneumonitis (RP), in lung cancer patients.

Methods.

A total of 160 lung cancer patients, who were diagnosed with inoperable lung cancer and received radiotherapy, were included in the present study from September 2009 to December 2011.

TaqMan Real-Time PCR (RT-PCR) was used to verify the SNPs of LIG4 and HSPB1 genes.

Chi-square criterion was used to compare the differences in demographic characteristics, exposure to risk factors, and SNPs genotypes.

Crude odds ratios (ORs) with 95% confidence intervals (95% CI) were calculated by logistic regression analysis.

All statistical analyses were conducted in SPSS 18.0.

Results.

A total of 32 (20.0%) lung cancer patients had RP after receiving radiotherapy.

Of the 32 cases, 4 cases were of grade 2, 24 cases were of grade 3, and 4 cases were of grade 4.

However, our results indicated that the general condition and treatment of all patients had no significant difference with RP risk ( P > 0.0 5 ) .

Meanwhile, our results revealed that there was no significant association between the frequencies of LIG4 rs1805388 and HSPB1 rs2868371 genotype distribution and the risk of RP ( P > 0.05 ) .

Conclusion.

In conclusion, we demonstrated that the genetic polymorphisms in LIG4 rs1805388 and HSPB1 rs2868371 were not obviously correlated with the risk of RP and RILI of lung cancer.