MicroRNA Promoter Identification in Arabidopsis Using Multiple Histone Markers
Joint Authors
Wang, Fang
Zhao, Yuming
Juan, Liran
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A microRNA is a small noncoding RNA molecule, which functions in RNA silencing and posttranscriptional regulation of gene expression.
To understand the mechanism of the activation of microRNA genes, the location of promoter regions driving their expression is required to be annotated precisely.
Only a fraction of microRNA genes have confirmed transcription start sites (TSSs), which hinders our understanding of the transcription factor binding events.
With the development of the next generation sequencing technology, the chromatin states can be inferred precisely by virtue of a combination of specific histone modifications.
Using the genome-wide profiles of nine histone markers including H3K4me2, H3K4me3, H3K9Ac, H3K9me2, H3K18Ac, H3K27me1, H3K27me3, H3K36me2, and H3K36me3, we developed a computational strategy to identify the promoter regions of most microRNA genes in Arabidopsis, based upon the assumption that the distribution of histone markers around the TSSs of microRNA genes is similar to the TSSs of protein coding genes.
Among 298 miRNA genes, our model identified 42 independent miRNA TSSs and 132 miRNA TSSs, which are located in the promoters of upstream genes.
The identification of promoters will provide better understanding of microRNA regulation and can play an important role in the study of diseases at genetic level.
American Psychological Association (APA)
Zhao, Yuming& Wang, Fang& Juan, Liran. 2015. MicroRNA Promoter Identification in Arabidopsis Using Multiple Histone Markers. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057046
Modern Language Association (MLA)
Zhao, Yuming…[et al.]. MicroRNA Promoter Identification in Arabidopsis Using Multiple Histone Markers. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057046
American Medical Association (AMA)
Zhao, Yuming& Wang, Fang& Juan, Liran. MicroRNA Promoter Identification in Arabidopsis Using Multiple Histone Markers. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057046
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057046