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Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiense in Urine and Saliva Samples inNonhuman Primate Model
Joint Authors
Ngotho, J. M.
Kagira, John Maina
Gachie, Beatrice Muthoni
Waema, Maxwell Wambua
Maina, Naomi Wangari
Nyawira Maranga, Dawn
Muturi Karanja, Simon
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease.
The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa.
Early diagnosis is hampered by lack of sensitive diagnostic techniques.
This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T.
b.
gambiense infection in a vervet monkey HAT model.
Six vervet monkeys were experimentally infected with T.
b.
gambiense IL3253 and monitored for 180 days after infection.
Parasitaemia was scored daily.
Blood, cerebrospinal fluid (CSF), saliva, and urine samples were collected weekly.
PCR and LAMP were performed on serum, CSF, saliva, and urine samples.
The detection by LAMP was significantly higher than that of parasitological methods and PCR in all the samples.
The performance of LAMP varied between the samples and was better in serum followed by saliva and then urine samples.
In the saliva samples, LAMP had 100% detection between 21 and 77 dpi, whereas in urine the detection it was slightly lower, but there was over 80% detection between 28 and 91 dpi.
However, LAMP could not detect trypanosomes in either saliva or urine after 140 and 126 dpi, respectively.
The findings of this study emphasize the importance of LAMP in diagnosis of HAT using saliva and urine samples.
American Psychological Association (APA)
Ngotho, J. M.& Kagira, John Maina& Gachie, Beatrice Muthoni& Muturi Karanja, Simon& Waema, Maxwell Wambua& Nyawira Maranga, Dawn…[et al.]. 2015. Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiense in Urine and Saliva Samples inNonhuman Primate Model. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1057071
Modern Language Association (MLA)
Ngotho, J. M.…[et al.]. Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiense in Urine and Saliva Samples inNonhuman Primate Model. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1057071
American Medical Association (AMA)
Ngotho, J. M.& Kagira, John Maina& Gachie, Beatrice Muthoni& Muturi Karanja, Simon& Waema, Maxwell Wambua& Nyawira Maranga, Dawn…[et al.]. Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiense in Urine and Saliva Samples inNonhuman Primate Model. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1057071
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057071