Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells
Joint Authors
Yu, Bin
Li, Runguang
Liang, Liang
Dou, Yonggang
Huang, Zeping
Mo, Huiting
Wang, Yaning
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study examined the effects of mechanical strain on osteogenic and adipogenic differentiation of cultured MSCs by stimulating MSCs cultured in general and adipogenic differentiation media using a mechanical strain device.
Markers of osteogenic (Runx2, Osx, and I-collagen) and adipogenic (PPARγ-2, C/EBPα, and lipid droplets) differentiation were examined using real-time PCR, western blot, immunocytochemical, or histochemical stain analyses.
Levels of Runx2 and Osx gradually increased in MSC groups in general medium subject to strain stimulation, as compared with in unstrained groups.
After adding the stress signal, I-collagen protein levels of expression were obviously promoted in cells in comparison to the controls.
The levels of PPARγ-2 and C/EBPα were decreased, and the emergence of lipid droplets was delayed in MSCs groups in adipogenic differentiation medium subject to strain stimulation, as compared with in unstrained groups.
Mechanical strain can promote differentiation of MSCs into osteoblasts and can impede differentiation into adipocytes.
These results clarify the mechanisms underlying the effects of exercise on bone repair and reconstruction and provide a more adequate scientific basis for the use of exercise therapy in the treatment of obesity and metabolic osteoporosis.
American Psychological Association (APA)
Li, Runguang& Liang, Liang& Dou, Yonggang& Huang, Zeping& Mo, Huiting& Wang, Yaning…[et al.]. 2015. Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057096
Modern Language Association (MLA)
Li, Runguang…[et al.]. Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057096
American Medical Association (AMA)
Li, Runguang& Liang, Liang& Dou, Yonggang& Huang, Zeping& Mo, Huiting& Wang, Yaning…[et al.]. Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057096