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454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature
Joint Authors
Gonzalez-Martinez, A.
Gonzalez-Lopez, J.
Rodriguez-Sanchez, Alejandro
Rodelas, Belén
Abbas, Ben A.
Martinez-Toledo, Maria Victoria
van Loosdrecht, Mark C. M.
Osorio, F.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for the Bacteria domain able to amplify the anammox 16S rRNA genes.
A metagenomic analysis (pyrosequencing) of total bacterial diversity including anammox population in five autotrophic nitrogen removal technologies, two bench-scale models (MBR and Low Temperature CANON) and three full-scale bioreactors (anammox, CANON, and DEMON), was successfully carried out by optimization of primer selection and PCR conditions (annealing temperature).
The universal primer 530F was identified as the best candidate for total bacteria and anammox bacteria diversity coverage.
Salt-adjusted optimum annealing temperature of primer 530F was calculated (47°C) and hence a range of annealing temperatures of 44–49°C was tested.
Pyrosequencing data showed that annealing temperature of 45°C yielded the best results in terms of species richness and diversity for all bioreactors analyzed.
American Psychological Association (APA)
Gonzalez-Martinez, A.& Rodriguez-Sanchez, Alejandro& Rodelas, Belén& Abbas, Ben A.& Martinez-Toledo, Maria Victoria& van Loosdrecht, Mark C. M.…[et al.]. 2015. 454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1057132
Modern Language Association (MLA)
Gonzalez-Martinez, A.…[et al.]. 454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1057132
American Medical Association (AMA)
Gonzalez-Martinez, A.& Rodriguez-Sanchez, Alejandro& Rodelas, Belén& Abbas, Ben A.& Martinez-Toledo, Maria Victoria& van Loosdrecht, Mark C. M.…[et al.]. 454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1057132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057132