Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma

Abstract EN

This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP).

Seven healthy gelding crossbred horses aged 16 to 17 years were used.

Two rectangle-shaped wounds were created surgically in the right and left gluteal regions.

Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions.

The contralateral region was used as control (CG).

Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2).

The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively.

In the scar of the treated group, COL III showed important (p<0.05) increase in immunoreaction in T2 compared with T1.

The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III.

However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.