Absence of the mecA Gene in Methicillin Resistant Staphylococcus aureus Isolated from Different Clinical Specimens in Shendi City, Sudan
Joint Authors
Elhassan, Mogahid M.
Ozbak, Hani A.
Hemeg, Hassan A.
Elmekki, Miskelyemen A.
Ahmed, Leila M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Absolute dependence on mecA gene as the defining standard in determining the resistance of S.
aureus to methicillin became the subject of distrust by many researchers.
The present study aimed to determine the frequency of mecA gene in methicillin resistant S.
aureus (MRSA) isolates using polymerase chain reaction and to correlate its presence to conventional method.
In this regard, two hundred S.
aureus isolates were collected from patients with different diseases attending different hospitals in Shandi City, Sudan.
Phenotypic Kirby-Bauer method confirmed the existence of methicillin resistant S.
aureus in 61.5% of the subjected isolates with MICs ranging from 4 μg/mL to 256 μg/mL when using E-test.
However, when amplifying a 310 bp fragment of the mecA gene by PCR, twelve out of the 123 MRSA isolates (9.8%) were mecA negative, whereas all the 77 methicillin sensitive S.
aureus (MSSA) were mecA negative.
In conclusion, this study drew attention to the credibility of the mecA gene and its usefulness in the detection of all MRSA strains without referring to the traditional methods.
Hence, it is highly recommended to consider alternative mechanisms for β-lactam resistance that may compete with mecA gene in the emergence of MRSA phenomenon in the community.
American Psychological Association (APA)
Elhassan, Mogahid M.& Ozbak, Hani A.& Hemeg, Hassan A.& Elmekki, Miskelyemen A.& Ahmed, Leila M.. 2015. Absence of the mecA Gene in Methicillin Resistant Staphylococcus aureus Isolated from Different Clinical Specimens in Shendi City, Sudan. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1057150
Modern Language Association (MLA)
Elhassan, Mogahid M.…[et al.]. Absence of the mecA Gene in Methicillin Resistant Staphylococcus aureus Isolated from Different Clinical Specimens in Shendi City, Sudan. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1057150
American Medical Association (AMA)
Elhassan, Mogahid M.& Ozbak, Hani A.& Hemeg, Hassan A.& Elmekki, Miskelyemen A.& Ahmed, Leila M.. Absence of the mecA Gene in Methicillin Resistant Staphylococcus aureus Isolated from Different Clinical Specimens in Shendi City, Sudan. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1057150
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057150