A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma
Joint Authors
Ip, Joseph Chok Yan
Ko, Josephine Mun Yee
Yu, Valen Zhuoyou
Chan, Kwok Wah
Lam, Alfred K.
Law, Simon
Tong, Daniel King Hung
Lung, Maria Li
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Increasing evidence indicates tumor-stromal interactions play a crucial role in cancer.
An in vivo esophageal squamous cell carcinoma (ESCC) orthotopic animal model was developed with bioluminescence imaging established with a real-time monitoring platform for functional and signaling investigation of tumor-stromal interactions.
The model was produced by injection of luciferase-labelled ESCC cells into the intraesophageal wall of nude mice.
Histological examination indicates this orthotopic model is highly reproducible with 100% tumorigenesis among the four ESCC cell lines tested.
This new model recapitulates many clinical and pathological properties of human ESCC, including esophageal luminal stricture by squamous cell carcinoma with nodular tumor growth, adventitia invasion, lymphovascular invasion, and perineural infiltration.
It was tested using an AKT shRNA knockdown of ESCC cell lines and the in vivo tumor suppressive effects of AKT knockdown were observed.
In conclusion, this ESCC orthotopic mouse model allows investigation of gene functions of cancer cells in a more natural tumor microenvironment and has advantages over previous established models.
It provides a versatile platform with potential application for metastasis and therapeutic regimen testing.
American Psychological Association (APA)
Ip, Joseph Chok Yan& Ko, Josephine Mun Yee& Yu, Valen Zhuoyou& Chan, Kwok Wah& Lam, Alfred K.& Law, Simon…[et al.]. 2015. A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057205
Modern Language Association (MLA)
Ip, Joseph Chok Yan…[et al.]. A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057205
American Medical Association (AMA)
Ip, Joseph Chok Yan& Ko, Josephine Mun Yee& Yu, Valen Zhuoyou& Chan, Kwok Wah& Lam, Alfred K.& Law, Simon…[et al.]. A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057205