Comparison of Different Strategies for SelectionAdaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel
Joint Authors
Varrone, C.
Heggeset, T. M. B.
Le, S. B.
Haugen, T.
Markussen, S.
Skiadas, I. V.
Gavala, H. N.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Objective of this study was the selection and adaptation of mixed microbial cultures (MMCs), able to ferment crude glycerol generated from animal fat-based biodiesel and produce building-blocks and green chemicals.
Various adaptation strategies have been investigated for the enrichment of suitable and stable MMC, trying to overcome inhibition problems and enhance substrate degradation efficiency, as well as generation of soluble fermentation products.
Repeated transfers in small batches and fed-batch conditions have been applied, comparing the use of different inoculum, growth media, and Kinetic Control.
The adaptation of activated sludge inoculum was performed successfully and continued unhindered for several months.
The best results showed a substrate degradation efficiency of almost 100% (about 10 g/L glycerol in 21 h) and different dominant metabolic products were obtained, depending on the selection strategy (mainly 1,3-propanediol, ethanol, or butyrate).
On the other hand, anaerobic sludge exhibited inactivation after a few transfers.
To circumvent this problem, fed-batch mode was used as an alternative adaptation strategy, which led to effective substrate degradation and high 1,3-propanediol and butyrate production.
Changes in microbial composition were monitored by means of Next Generation Sequencing, revealing a dominance of glycerol consuming species, such as Clostridium, Klebsiella, and Escherichia.
American Psychological Association (APA)
Varrone, C.& Heggeset, T. M. B.& Le, S. B.& Haugen, T.& Markussen, S.& Skiadas, I. V.…[et al.]. 2015. Comparison of Different Strategies for SelectionAdaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1057292
Modern Language Association (MLA)
Varrone, C.…[et al.]. Comparison of Different Strategies for SelectionAdaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1057292
American Medical Association (AMA)
Varrone, C.& Heggeset, T. M. B.& Le, S. B.& Haugen, T.& Markussen, S.& Skiadas, I. V.…[et al.]. Comparison of Different Strategies for SelectionAdaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1057292
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057292