Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications
Joint Authors
Adnan, Mohd
Lohani, Mohtashim
Haque, Shafiul
Alshammari, Eyad M. A.
Khan, Saif
Jawed, Arshad
Khan, Mahvish
Nabi, Gowher
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ovotransferrin is the second most abundant protein (~12-13% of the total egg protein) in egg white after ovalbumin.
Ovotransferrin is a potent natural antimicrobial agent as it possesses antibacterial, antifungal, and antiviral properties and is also the major metal binding protein found in egg, which makes it an industrially important protein.
Ovotransferrin was extracted from egg white using its metal (iron) binding properties.
In the present study, eggs from two different sources were used (fresh local eggs from domestic household source and poultry eggs from shops) to compare the results and Response Surface Methodology was used for the experiment design and data analysis.
The following extraction conditions were optimized so as to maximize the yield of ovotransferrin from egg white: ethanol % (v/v) and pH and volume (mL) of 25 mM FeCl3/50 mL of egg white.
A maximum yield of ~85 ± 2.5% was obtained near the optimum extraction conditions.
The yield was calculated based on the theoretical value (934 mg) of ovotransferrin in 100 mL of 1.5x diluted egg white solution.
Our results suggest that efficient downstream processing may reduce the cost of overall production process of this promising enzyme, making it a natural and cost-effective alternative to the existing chemically synthesized antimicrobial agents.
American Psychological Association (APA)
Alshammari, Eyad M. A. & Khan, Saif& Jawed, Arshad& Adnan, Mohd& Khan, Mahvish& Nabi, Gowher…[et al.]. 2015. Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057299
Modern Language Association (MLA)
Alshammari, Eyad M. A.…[et al.]. Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057299
American Medical Association (AMA)
Alshammari, Eyad M. A. & Khan, Saif& Jawed, Arshad& Adnan, Mohd& Khan, Mahvish& Nabi, Gowher…[et al.]. Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057299
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057299