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Detection of Tropical Fungi in Formalin-Fixed, Paraffin-Embedded Tissue: Still an Indication for Microscopy in Times of Sequence-Based Diagnosis?
Joint Authors
Frickmann, Hagen
Hagen, Ralf Matthias
Loderstaedt, Ulrike
Racz, Paul
Tenner-Racz, Klara
Eggert, Petra
Haeupler, Alexandra
Bialek, Ralf
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Introduction.
The aim of the study was the evaluation of panfungal PCR protocols with subsequent sequence analysis for the diagnostic identification of invasive mycoses in formalin-fixed, paraffin-embedded tissue samples with rare tropical mycoses.
Materials and Methods.
Five different previously described panfungal PCR/sequencing protocols targeting 18S and 28S ribosomal RNA gene fragments as well as internal transcribed spacer 1 and 2 fragments were evaluated with a collection of 17 formalin-fixed, paraffin-embedded tissue samples of patients with rare and/or tropical invasive mycoses, comprising chromoblastomycosis, coccidioidomycosis, cryptococcosis, histoplasmosis, mucormycosis, mycetoma/maduromycosis, and rhinosporidiosis, in a proof-of-principle analysis.
Results.
The primers of the panfungal PCRs readily and predominantly reacted with contaminating environmental fungi that had deposited on the paraffin blocks.
Altogether three sequence results of histoplasmosis and mycetoma samples that matched the histological assessment were associated with sample age <10 years and virtually without PCR inhibition.
Conclusions.
The high risk of amplifying environmental contaminants severely reduces the usefulness of the assessed panfungal PCR/sequencing protocols for the identification of rare and/or tropical mycoses in stored formalin-fixed, paraffin-embedded tissues.
Histological assessment remains valuable for such indications if cultural differentiation is impossible from inactivated sample material.
American Psychological Association (APA)
Frickmann, Hagen& Loderstaedt, Ulrike& Racz, Paul& Tenner-Racz, Klara& Eggert, Petra& Haeupler, Alexandra…[et al.]. 2015. Detection of Tropical Fungi in Formalin-Fixed, Paraffin-Embedded Tissue: Still an Indication for Microscopy in Times of Sequence-Based Diagnosis?. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1057308
Modern Language Association (MLA)
Frickmann, Hagen…[et al.]. Detection of Tropical Fungi in Formalin-Fixed, Paraffin-Embedded Tissue: Still an Indication for Microscopy in Times of Sequence-Based Diagnosis?. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1057308
American Medical Association (AMA)
Frickmann, Hagen& Loderstaedt, Ulrike& Racz, Paul& Tenner-Racz, Klara& Eggert, Petra& Haeupler, Alexandra…[et al.]. Detection of Tropical Fungi in Formalin-Fixed, Paraffin-Embedded Tissue: Still an Indication for Microscopy in Times of Sequence-Based Diagnosis?. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1057308
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057308