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IFN-CSP Inhibiting Hepatitis B Virus in HepG2.2.15 Cells Involves JAK-STAT Signal Pathway
Joint Authors
Lu, Xuemei
Wang, Jie
Huang, Yanting
Zeng, Wenting
Jin, Xiaobao
Zhu, Jiayong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Frequent and high-dose administration of interferon to patients with viral hepatitis results in various side effects.
In our previous study, a novel liver-targeting interferon (IFN-CSP) combining Plasmodium region I peptide with IFNα2b was successfully designed and expressed in the Escherichia coli expression systems.
This targeting would target the IFNα2b specifically to the liver, thus reducing the adverse events.
In the present study, we further investigated the anti-HBV effects and molecular mechanisms of recombinant IFN-CSP in HepG2.2.15 cell line.
Hepatitis B surface antigen (HBsAg) and HBe antigen (HBeAg) in the culture supernatants were analyzed by enzyme-linked immunosorbent assay (ELISA).
HBV-DNA was measured by real-time quantitative PCR.
HBV core protein was assayed by immunofluorescent and western blot analysis.
The expressions of signal transducers and transactivator 1 (STAT1), STAT2, IFN regulatory factor 9 (IRF-9), and 2′-5′-oligoadenylate synthetase 1 (OAS1) were investigated by the reverse transcription PCR and western blot analysis.
Results indicate IFN-CSP efficiently inhibited HBsAg and HBeAg secretion, HBV-DNA replication, and HBV core protein expression in HepG2.2.15 cells.
The anti-HBV mechanisms involve activation of JAK-STAT signaling and increase of the anti-HBV protein OAS expression.
IFN-CSP could be a good substitute for IFNα2b for anti-HBV treatment.
American Psychological Association (APA)
Lu, Xuemei& Wang, Jie& Jin, Xiaobao& Huang, Yanting& Zeng, Wenting& Zhu, Jiayong. 2015. IFN-CSP Inhibiting Hepatitis B Virus in HepG2.2.15 Cells Involves JAK-STAT Signal Pathway. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1057384
Modern Language Association (MLA)
Lu, Xuemei…[et al.]. IFN-CSP Inhibiting Hepatitis B Virus in HepG2.2.15 Cells Involves JAK-STAT Signal Pathway. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1057384
American Medical Association (AMA)
Lu, Xuemei& Wang, Jie& Jin, Xiaobao& Huang, Yanting& Zeng, Wenting& Zhu, Jiayong. IFN-CSP Inhibiting Hepatitis B Virus in HepG2.2.15 Cells Involves JAK-STAT Signal Pathway. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1057384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057384