Effects of Chronic REM Sleep Restriction on D 1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex
Joint Authors
Han, Yan
Wen, Xiaosa
Rong, Fei
Chen, Xinmin
Ouyang, Ruying
Wu, Shuai
Nian, Hua
Ma, Wenling
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The prefrontal cortex (PFC) mediates cognitive function that is sensitive to disruption by sleep loss, and molecular mechanisms regulating neural dysfunction induced by chronic sleep restriction (CSR), particularly in the PFC, have yet to be completely understood.
The aim of the present study was to investigate the effect of chronic REM sleep restriction (REM-CSR) on the D1 receptor (D1R) and key molecules in D1R’ signal pathways in PFC.
We employed the modified multiple platform method to create the REM-CSR rat model.
The ultrastructure of PFC was observed by electron microscopy.
HPLC was performed to measure the DA level in PFC.
The expressions of genes and proteins of related molecules were assayed by real-time PCR and Western blot, respectively.
The general state and morphology of PFC in rats were changed by CSR, and DA level and the expression of D1R in PFC were markedly decreased ( P < 0.01 , P < 0.05 ); the expression of phosphor-PKAcα was significantly lowered in CSR rats ( P < 0.05 ).
The present results suggested that the alteration of neuropathology and D1R expression in PFC may be associated with CSR induced cognitive dysfunction, and the PKA pathway of D1R may play an important role in the impairment of advanced neural function.
American Psychological Association (APA)
Han, Yan& Wen, Xiaosa& Rong, Fei& Chen, Xinmin& Ouyang, Ruying& Wu, Shuai…[et al.]. 2015. Effects of Chronic REM Sleep Restriction on D 1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057466
Modern Language Association (MLA)
Han, Yan…[et al.]. Effects of Chronic REM Sleep Restriction on D 1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1057466
American Medical Association (AMA)
Han, Yan& Wen, Xiaosa& Rong, Fei& Chen, Xinmin& Ouyang, Ruying& Wu, Shuai…[et al.]. Effects of Chronic REM Sleep Restriction on D 1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057466
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057466