Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism
Joint Authors
Xu, Jian-xin
Song, Hai-ping
Bu, Qing-Xia
Feng, De-Peng
Xu, Xiao-Fan
Sun, Qian-Ru
Li, Xue-Li
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism.
The subjects were PC12 cells.
They were randomly divided into six groups: control, MPP+ (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP+ (250 μmol/L), Z-YVAD-CHO (10 nM) + MPP+ group, and Z-DEVD-CHO (10 nM) + MPP+ group.
Cell viability was measured by MTT methods; the content of tyrosine hydroxylase was measured by immunocytochemistry method of avidinbiotin peroxidase complex; apoptosis ratio was measured by flow cytometry.
The results showed that cell viability in the MPP+ group was lower than in all other five groups.
There was no difference in cell viability between isoflavone + MPP+ and control group.
Optical density of TH positive cells in isoflavone group was higher than in control, isoflavone + MPP+, and MPP+ only groups.
The apoptosis ratio in the isoflavone + MPP+ group and control group and the Z-YVAD-CHO + MPP+ and Z-DEVD-CHO + MPP+ group was similar, which was lower than in the MPP+ group.
The lowest apoptosis ratio was found in the isoflavone only group.
American Psychological Association (APA)
Xu, Jian-xin& Song, Hai-ping& Bu, Qing-Xia& Feng, De-Peng& Xu, Xiao-Fan& Sun, Qian-Ru…[et al.]. 2015. Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism. Behavioural Neurology،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057571
Modern Language Association (MLA)
Xu, Jian-xin…[et al.]. Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism. Behavioural Neurology No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1057571
American Medical Association (AMA)
Xu, Jian-xin& Song, Hai-ping& Bu, Qing-Xia& Feng, De-Peng& Xu, Xiao-Fan& Sun, Qian-Ru…[et al.]. Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism. Behavioural Neurology. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1057571
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057571