Antidiabetic Activity of Ruellia tuberosa L., Role of α-Amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches
Joint Authors
Ratna Wulan, Dyah
Priyo Utomo, Edi
Mahdi, Chanif
Source
Biochemistry Research International
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Ruellia tuberosa L.
is a folk remedy in the treatment of diabetes mellitus.
However, its hypoglycemic activity has not been investigated so far.
In the present study, the antidiabetic mechanism of the n-hexane fraction of methanolic extract (HFME) of this plant was investigated in silico, in vitro, and in vivo.
In silico study was performed using AutoDock4.2 software.
In vitro α-amylase inhibitory activity was investigated by starch-iodine method.
A single dose of 450 mg/kg HFME for 14 days was subjected to an antidiabetic screening in vivo by a multiple low dose streptozotocin (MLD-STZ) induced rats.
Molecular modeling results show that Betulin exhibited noncompetitive α-amylase inhibitory activities.
The effect of HFME elicited significant reductions of diabetic rat blood glucose.
A single dose administration of HFME inhibited α-amylase activity in vivo ( P < 0.01 ) compared to a diabetic control group.
Moreover, this extract strongly inhibited the α-amylase activity in vitro (IC50 0.14 ± 0.005 mg/mL).
It is concluded that HFME exerted an antidiabetic effect via α-amylase inhibitor.
Our findings provide a possible hypoglycemic action of R.
tuberosa L.
as an alternative therapy in the management of diabetes.
American Psychological Association (APA)
Ratna Wulan, Dyah& Priyo Utomo, Edi& Mahdi, Chanif. 2015. Antidiabetic Activity of Ruellia tuberosa L., Role of α-Amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches. Biochemistry Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057611
Modern Language Association (MLA)
Ratna Wulan, Dyah…[et al.]. Antidiabetic Activity of Ruellia tuberosa L., Role of α-Amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches. Biochemistry Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1057611
American Medical Association (AMA)
Ratna Wulan, Dyah& Priyo Utomo, Edi& Mahdi, Chanif. Antidiabetic Activity of Ruellia tuberosa L., Role of α-Amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches. Biochemistry Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057611