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Bp-13 PLA2: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom
Joint Authors
Torres-Huaco, Frank Denis
Sucasaca-Monzón, Georgina
Randazzo-Moura, Priscila
Rocha, Thalita
Vilca-Quispe, Augusto
Rodrigues-Simioni, Léa
Ponce Soto, L. A.
Marangoni, Sérgio
da Cruz-Höfling, Maria Alice
Source
Biochemistry Research International
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A new PLA2 (Bp-13) was purified from Bothrops pauloensis snake venom after a single chromatographic step of RP-HPLC on μ-Bondapak C-18.
Amino acid analysis showed a high content of hydrophobic and basic amino acids and 14 half-cysteine residues.
The N-terminal sequence showed a high degree of homology with basic Asp49 PLA2 myotoxins from other Bothrops venoms.
Bp-13 showed allosteric enzymatic behavior and maximal activity at pH 8.1, 36°–45°C.
Full Bp-13 PLA2 activity required Ca2+; its PLA2 activity was inhibited by Mg2+, Mn2+, Sr2+, and Cd2+ in the presence and absence of 1 mM Ca2+.
In the mouse phrenic nerve-diaphragm (PND) preparation, the time for 50% paralysis was concentration-dependent (P<0.05).
Both the replacement of Ca2+ by Sr2+ and temperature lowering (24°C) inhibited the Bp-13 PLA2-induced twitch-tension blockade.
Bp-13 PLA2 inhibited the contractile response to direct electrical stimulation in curarized mouse PND preparation corroborating its contracture effect.
In biventer cervicis preparations, Bp-13 induced irreversible twitch-tension blockade and the KCl evoked contracture was partially, but significantly, inhibited (P>0.05).
The main effect of this new Asp49 PLA2 of Bothrops pauloensis venom is on muscle fiber sarcolemma, with avian preparation being less responsive than rodent preparation.
The study enhances biochemical and pharmacological characterization of B.
pauloensis venom.
American Psychological Association (APA)
Sucasaca-Monzón, Georgina& Randazzo-Moura, Priscila& Rocha, Thalita& Torres-Huaco, Frank Denis& Vilca-Quispe, Augusto& Ponce Soto, L. A.…[et al.]. 2015. Bp-13 PLA2: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom. Biochemistry Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1057624
Modern Language Association (MLA)
Sucasaca-Monzón, Georgina…[et al.]. Bp-13 PLA2: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom. Biochemistry Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1057624
American Medical Association (AMA)
Sucasaca-Monzón, Georgina& Randazzo-Moura, Priscila& Rocha, Thalita& Torres-Huaco, Frank Denis& Vilca-Quispe, Augusto& Ponce Soto, L. A.…[et al.]. Bp-13 PLA2: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom. Biochemistry Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1057624
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057624