High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research
Joint Authors
Xi, Yan
Lin, Xiaojie
Yuan, Falei
Yang, Guo-Yuan
Zhao, Jun
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Imaging techniques for visualizing cerebral vasculature and distinguishing functional areas are essential and critical to the study of various brain diseases.
In this paper, with the X-ray phase-contrast imaging technique, we proposed an experiment scheme for the ex vivo mouse brain study, achieving both high spatial resolution and improved soft-tissue contrast.
This scheme includes two steps: sample preparation and volume reconstruction.
In the first step, we use heparinized saline to displace the blood inside cerebral vessels and then replace it with air making air-filled mouse brain.
After sample preparation, X-ray phase-contrast tomography is performed to collect the data for volume reconstruction.
Here, we adopt a phase-retrieval combined filtered backprojection method to reconstruct its three-dimensional structure and redesigned the reconstruction kernel.
To evaluate its performance, we carried out experiments at Shanghai Synchrotron Radiation Facility.
The results show that the air-tissue structured cerebral vasculatures are highly visible with propagation-based phase-contrast imaging and can be clearly resolved in reconstructed cross-images.
Besides, functional areas, such as the corpus callosum, corpus striatum, and nuclei, are also clearly resolved.
The proposed method is comparable with hematoxylin and eosin staining method but represents the studied mouse brain in three dimensions, offering a potential powerful tool for the research of brain disorders.
American Psychological Association (APA)
Xi, Yan& Lin, Xiaojie& Yuan, Falei& Yang, Guo-Yuan& Zhao, Jun. 2015. High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research. Computational and Mathematical Methods in Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1057927
Modern Language Association (MLA)
Xi, Yan…[et al.]. High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research. Computational and Mathematical Methods in Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1057927
American Medical Association (AMA)
Xi, Yan& Lin, Xiaojie& Yuan, Falei& Yang, Guo-Yuan& Zhao, Jun. High-Resolution and Quantitative X-Ray Phase-Contrast Tomography for Mouse Brain Research. Computational and Mathematical Methods in Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1057927
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057927