Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences
Joint Authors
Wu, Jian
Xu, Yingke
Feng, Zhouyan
Zheng, Xiao-xiang
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Quantitative analysis of the dynamic behavior about membrane-bound secretory vesicles has proven to be important in biological research.
This paper proposes a novel approach to automatically identify the elusive fusion events between VAMP2-pHluorin labeled GLUT4 storage vesicles (GSVs) and the plasma membrane.
The differentiation is implemented to detect the initiation of fusion events by modified forward subtraction of consecutive frames in the TIRFM image sequence.
Spatially connected pixels in difference images brighter than a specified adaptive threshold are grouped into a distinct fusion spot.
The vesicles are located at the intensity-weighted centroid of their fusion spots.
To reveal the true in vivo nature of a fusion event, 2D Gaussian fitting for the fusion spot is used to derive the intensity-weighted centroid and the spot size during the fusion process.
The fusion event and its termination can be determined according to the change of spot size.
The method is evaluated on real experiment data with ground truth annotated by expert cell biologists.
The evaluation results show that it can achieve relatively high accuracy comparing favorably to the manual analysis, yet at a small fraction of time.
American Psychological Association (APA)
Wu, Jian& Xu, Yingke& Feng, Zhouyan& Zheng, Xiao-xiang. 2015. Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences. Computational and Mathematical Methods in Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1057953
Modern Language Association (MLA)
Wu, Jian…[et al.]. Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences. Computational and Mathematical Methods in Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1057953
American Medical Association (AMA)
Wu, Jian& Xu, Yingke& Feng, Zhouyan& Zheng, Xiao-xiang. Automatically Identifying Fusion Events between GLUT4 Storage Vesicles and the Plasma Membrane in TIRF Microscopy Image Sequences. Computational and Mathematical Methods in Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1057953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057953