Inhibition of HIF-1α Affects Autophagy Mediated Glycosylation in Oral Squamous Cell Carcinoma Cells

Abstract EN

Purpose.

To validate the function of autophagy with the regulation of hypoxia inhibitor-induced glycosylation in oral squamous cell carcinoma cell.

Methods.

Human Tca8113 cell line was used to detect autophagy and glycosylation related protein expression by western blotting and immunofluorescence with HIF-1α inhibitor.

Short interfering RNA (siRNA) transfection blocked human ATG12 and ATG1.

Results.

HIF-1α inhibitor PX-478 reduced the amount of LC3-II and LC3-I in Tca8113 cells.

PX-478 decreased the expression of O-GlcNAc and OGT and increased OGA expression.

The tendency of O-GlcNAc showed a similar pattern to OGT.

PX-478 gradually decreased OGT expression in Tca8113 cells.

Protein level of O-GlcNAc and OGT increased in ATG12 and ATG1 depletion.

The expression of OGT decreased at first and then rose slowly with the treatment of Atg12 and Atg1 siRNA and PX-478 fluctuant.

Autophagy affected the stability of OGT when HIF-1α signaling was blocked.

Conclusions.

Autophagy reduced by hypoxic stress inhibited.

HIF-1α inhibitor decreased glycosylation.

OGT became unstable in the absence of autophagy when HIF-1α signaling was blocked.