Hypermethylation-Associated Silencing of miR-125a and miR-125b: A Potential Marker in Colorectal Cancer
Joint Authors
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
MicroRNAs (miRNAs) have been found to be downregulated in human colorectal cancer (CRC), and some of them may function as tumor suppressor genes (TSGs).
Aberrant methylation triggers the inactivation of TSGs during tumorigenesis.
Patients and Methods.
We investigated the methylation status of miR-125 family in CRC tissues and adjacent nontumor tissues by using bisulfite sequencing PCR (BSP).
The expression levels of the two miRNAs were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
Results.
The methylation frequency of miR-125a and miR-125b was higher in CRC tissues.
QRT-PCR analysis showed that miR-125a and miR-125b were significantly downregulated in CRC tissues.
Moreover, the expression levels of miR-125a and miR-125b were inversely correlated to CpG island methylation in CRC.
Conclusions.
Our results suggest that DNA hypermethylation may be involved in the inactivation of miR-125a and miR-125b in CRC, and hypermethylation of miR-125 is a potential biomarker for clinical outcome.
American Psychological Association (APA)
Chen, Hui& Xu, Zhiying. 2015. Hypermethylation-Associated Silencing of miR-125a and miR-125b: A Potential Marker in Colorectal Cancer. Disease Markers،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1060913
Modern Language Association (MLA)
Chen, Hui& Xu, Zhiying. Hypermethylation-Associated Silencing of miR-125a and miR-125b: A Potential Marker in Colorectal Cancer. Disease Markers No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1060913
American Medical Association (AMA)
Chen, Hui& Xu, Zhiying. Hypermethylation-Associated Silencing of miR-125a and miR-125b: A Potential Marker in Colorectal Cancer. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1060913
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1060913