Predictive Performance of a Seven-Plex Antibody Array in Prenatal Screening for Down Syndrome
Joint Authors
Zutt, Ilse
Koster, Maria P. H.
Imholz, Sandra
Pennings, Jeroen L. A.
Schielen, Peter C. J. I.
Siljee, Jacqueline E.
Rodenburg, Wendy
de Vries, Annemieke
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We evaluated the use of multiplex antibody array methodology for simultaneous measurement of serum protein markers for first trimester screening of Down Syndrome (DS) and other pregnancy outcomes such as preeclampsia.
For this purpose, we constructed an antibody array for indirect (“sandwich”) measurement of seven serum proteins: pregnancy-associated plasma protein-A (PAPP-A), free beta subunit of human chorionic gonadotropin (fβ-hCG), alpha-fetoprotein (AFP), angiopoietin-like 3 (ANGPTL3), epidermal growth factor (EGF), insulin-like growth factor 2 (IGFII), and superoxide dismutase 1 (SOD1).
This array was tested using 170 DS cases and 510 matched controls drawn during the 8th–13th weeks of pregnancy.
Data were used for prediction modelling and compared to previously obtained AutoDELFIA immunoassay data for PAPP-A and fβ-hCG.
PAPP-A and fβ-hCG serum concentrations obtained using antibody arrays were highly correlated with AutoDELFIA data.
Moreover, DS prediction modeling using (log-MoMmed) antibody array and AutoDELFIA data gave comparable results.
Of the other markers, AFP and IGFII showed significant changes in concentration, although adding these markers to a prediction model based on prior risk, PAPP-A and fβ-hCG did not improve the predictive performance.
We conclude that implementation of antibody arrays in a prenatal screening setting is feasible but will require additional first trimester screening markers.
American Psychological Association (APA)
Pennings, Jeroen L. A.& Imholz, Sandra& Zutt, Ilse& Koster, Maria P. H.& Siljee, Jacqueline E.& de Vries, Annemieke…[et al.]. 2015. Predictive Performance of a Seven-Plex Antibody Array in Prenatal Screening for Down Syndrome. Disease Markers،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1060967
Modern Language Association (MLA)
Pennings, Jeroen L. A.…[et al.]. Predictive Performance of a Seven-Plex Antibody Array in Prenatal Screening for Down Syndrome. Disease Markers No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1060967
American Medical Association (AMA)
Pennings, Jeroen L. A.& Imholz, Sandra& Zutt, Ilse& Koster, Maria P. H.& Siljee, Jacqueline E.& de Vries, Annemieke…[et al.]. Predictive Performance of a Seven-Plex Antibody Array in Prenatal Screening for Down Syndrome. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1060967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1060967