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The Prognostic Value of Pyrosequencing-Detected MGMT Promoter Hypermethylation in Newly Diagnosed Patients with Glioblastoma
Joint Authors
Pace, Andrea
Vidiri, A.
Carapella, C. M.
Villani, Veronica
Casini, Beatrice
Fabi, Alessandra
Prosperini, Luca
Carosi, M. A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
O6-methylguanine-DNA-methyltransferase (MGMT) has emerged as a relevant predictor of therapeutic response and good prognosis in patients with glioblastoma (GBM).
Transcriptionally active MGMT rapidly removes the alkyl adducts, preventing the formation of cross-links and thereby causing resistance to alkylating drugs.
Studies with pyrosequencing (PSQ) showed that this technique has a higher reproducibility and sensitivity than other techniques.
However, the definition of a prognostically relevant threshold for the percentage of MGMT methylation remains one of the most critical issues in the use of PSQ analysis.
The aim of this study was to define the cut-off value correlated with good favourable prognostic outcomes.
We retrospectively analyzed 51 patients (33 males, 18 females) with GBM who underwent surgery or biopsy.
The Receiver Operating Characteristics analysis showed that the best possible criteria for PSQ-detected percentage of MGMT methylation that predicted progression-free survival (PFS) and overall survival (OS) were 19% and 13%, respectively.
Patients with ≤19% of PSQ-detected MGMT had a shorter PFS (HR: 0.24, p<0.01); those ones with ≤13% had a shorter OS (HR: 0.33, p<0.05).
Our study reinforces the importance of MGMT in the management of GBM patients, but future studies with larger sample sizes are warranted to confirm our findings.
American Psychological Association (APA)
Villani, Veronica& Casini, Beatrice& Pace, Andrea& Prosperini, Luca& Carapella, C. M.& Vidiri, A.…[et al.]. 2015. The Prognostic Value of Pyrosequencing-Detected MGMT Promoter Hypermethylation in Newly Diagnosed Patients with Glioblastoma. Disease Markers،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1060989
Modern Language Association (MLA)
Villani, Veronica…[et al.]. The Prognostic Value of Pyrosequencing-Detected MGMT Promoter Hypermethylation in Newly Diagnosed Patients with Glioblastoma. Disease Markers No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1060989
American Medical Association (AMA)
Villani, Veronica& Casini, Beatrice& Pace, Andrea& Prosperini, Luca& Carapella, C. M.& Vidiri, A.…[et al.]. The Prognostic Value of Pyrosequencing-Detected MGMT Promoter Hypermethylation in Newly Diagnosed Patients with Glioblastoma. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1060989
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1060989