Comparative Proteomic Analysis of Human Cholangiocarcinoma Cell Lines: S100A2 as a Potential Candidate Protein Inducer of Invasion
Joint Authors
Roytrakul, Sittiruk
Wasuworawong, Kasima
Jindapornprasert, Kattaleeya
Komyod, Waraporn
Paemanee, Atchara
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Cholangiocarcinoma (CCA) is a bile duct cancer, commonly found in Asia including Thailand and especially in the northeastern region of Thailand.
To identify the proteins involved in carcinogenesis and metastasis of CCA, protein expression profiles of high-invasive KKU-M213 and low-invasive KKU-100 cell lines were compared using a comparative GeLC-MS/MS proteomics analysis.
A total of 651 differentially expressed proteins were detected of which 27 protein candidates were identified as having functions involved in cell motility.
A total of 22 proteins were significantly upregulated in KKU-M213, whereas 5 proteins were downregulated in KKU-M213.
S100A2, a calcium-binding protein in S100 protein family, is upregulated in KKU-M213.
S100A2 is implicated in metastasis development in several cancers.
The protein expression level of S100A2 was verified by Western blot analysis.
Intriguingly, high-invasive KKU-M213 cells showed higher expression of S100A2 than KKU-100 cells, consistent with proteomic data, suggesting that S100A2 may be a key protein involved in the progression of CCA.
However, the biological function of S100A2 in cholangiocarcinoma remains to be elucidated.
S100A2 might be a potential biomarker as well as a novel therapeutic target in CCA metastasis.
American Psychological Association (APA)
Wasuworawong, Kasima& Roytrakul, Sittiruk& Paemanee, Atchara& Jindapornprasert, Kattaleeya& Komyod, Waraporn. 2015. Comparative Proteomic Analysis of Human Cholangiocarcinoma Cell Lines: S100A2 as a Potential Candidate Protein Inducer of Invasion. Disease Markers،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1060996
Modern Language Association (MLA)
Wasuworawong, Kasima…[et al.]. Comparative Proteomic Analysis of Human Cholangiocarcinoma Cell Lines: S100A2 as a Potential Candidate Protein Inducer of Invasion. Disease Markers No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1060996
American Medical Association (AMA)
Wasuworawong, Kasima& Roytrakul, Sittiruk& Paemanee, Atchara& Jindapornprasert, Kattaleeya& Komyod, Waraporn. Comparative Proteomic Analysis of Human Cholangiocarcinoma Cell Lines: S100A2 as a Potential Candidate Protein Inducer of Invasion. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1060996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1060996