Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
Joint Authors
Li, Wen-han
Zhang, Hao
Guo, Qi
Wu, Xuan-di
Xu, Zi-sen
Dang, Cheng-xue
Xia, Peng
Song, Yong-chun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aim.
We examined the methylation status of SNCA and FBN1 genes in patients’ paired tissue and stool samples for detection of colorectal cancer (CRC).
Patients and Methods.
89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study.
In addition, 30 stool samples were collected as healthy controls.
Results.
The methylation level of those samples was measured by methylation-specific polymerase chain reaction (MSP).
The result shows that compared with the paired controls, both SNCA and FBN1 were significantly hypermethylated in CRC patients in tissue samples (P<0.001).
In the stool samples, hypermethylated SNCA and FBN1 were detected to be significantly higher than that in normal stool samples (P<0.001).
The combined sensitivity of at least one positive among the two markers in stool samples was 84.3%, with a specificity of 93.3%.
In addition, our experiment suggested that the positive rates of SNCA and FBN1 in Dukes A stage were significantly higher than that of FOBT (P=0.039; P=0.006, resp.).
Conclusion.
We concluded that methylation testing of SNCA and FBN1 genes in stool sample may offer a good alternative in a simple, promising, and noninvasive detection of colorectal cancer.
American Psychological Association (APA)
Li, Wen-han& Zhang, Hao& Guo, Qi& Wu, Xuan-di& Xu, Zi-sen& Dang, Cheng-xue…[et al.]. 2015. Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer. Disease Markers،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061005
Modern Language Association (MLA)
Li, Wen-han…[et al.]. Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer. Disease Markers No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1061005
American Medical Association (AMA)
Li, Wen-han& Zhang, Hao& Guo, Qi& Wu, Xuan-di& Xu, Zi-sen& Dang, Cheng-xue…[et al.]. Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061005