Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis
Joint Authors
Satoh, Kazumi
Yamada, Kazuo
Maniwa, Tomoko
Oda, Teiji
Matsumoto, Ken-ichi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Comprehensive analysis of proteome differentially expressed in response to surgery or drug treatment is useful to understand biological responses to dispensed interventions.
Here we investigated expression changes in sera of patients who suffered from calcific aortic stenosis (CAS), before and after surgery for aortic valve replacement.
Materials and Methods.
Sera obtained before and after surgery with depletion of highly abundant proteins were analyzed with iTRAQ labeling followed by nanoLC-MALDI-TOF/TOF-MS/MS.
Results.
Fifty-one proteins shared in five patients were identified with differential levels in postsurgical and presurgical sera.
Finally, 16 proteins that show statistically significant levels in patients’ sera compared with those in control sera ( P < 0.05 ) were identified.
Most of the identified proteins were positive acute-phase proteins.
Among three proteins other than acute-phase proteins, we confirmed increased levels of antithrombin-III and zinc-α-2-glycoprotein in postsurgical sera by Western blot analysis using other CAS patients’ sera.
Furthermore, antithrombin-III and zinc-α-2-glycoprotein were not found among proteins with differential levels in postsurgical and presurgical sera of patients with aortic aneurysms that we identified in a previous study.
Conclusions.
The results indicated that antithrombin-III and zinc-α-2-glycoprotein would become unique monitoring proteins for evaluating pathophysiological and biochemical processes occurring before and after surgery for CAS.
American Psychological Association (APA)
Satoh, Kazumi& Yamada, Kazuo& Maniwa, Tomoko& Oda, Teiji& Matsumoto, Ken-ichi. 2015. Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis. Disease Markers،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1061015
Modern Language Association (MLA)
Satoh, Kazumi…[et al.]. Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis. Disease Markers No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1061015
American Medical Association (AMA)
Satoh, Kazumi& Yamada, Kazuo& Maniwa, Tomoko& Oda, Teiji& Matsumoto, Ken-ichi. Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1061015
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061015