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miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G
Joint Authors
Sun, Huaibin
Tian, Jun
Xian, Wanhua
Xie, Tingting
Yang, Xiangdong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
miR-34a is a member of the miR-34 family and acts as a tumor suppressor in bladder cancer.
This study explored the regulative role of miR-34a on an orphan nuclear receptor HNF4G, which has a well-confirmed role in bladder tumor growth and invasion.
qRT-PCR analysis was applied to measure miR-34a expression in two tumorigenic bladder cancer cell lines 5637 and T24 and one normal human urothelial cell line SV-HUC-1.
Luciferase assay was performed to verify the putative binding between miR-34a and HNF4G.
The influence of miR-34a-HNF4G axis on cell viability, colony formation, and invasion was assessed with loss- and gain-of-function analysis.
This study observed that the miR-34a expressions in 5637 and T24 cells were significantly lower than in SV-HUC-1, while the muscle invasive cell sublines 5637-M and T24-M had even lower miR-34a expression than in the nonmuscle invasive sublines.
HNF4G has a 3′-UTR binding site with miR-34a and is a direct downstream target of miR-34a.
miR-34a can directly downregulate the expression of HNF4G and thus inhibit tumor cell viability, colony formation, and invasion.
Therefore, miR-34a-HNF4G axis is an important pathway modulating cell viability, proliferation, and invasion of bladder cancer cells.
American Psychological Association (APA)
Sun, Huaibin& Tian, Jun& Xian, Wanhua& Xie, Tingting& Yang, Xiangdong. 2015. miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G. Disease Markers،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061074
Modern Language Association (MLA)
Sun, Huaibin…[et al.]. miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G. Disease Markers No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061074
American Medical Association (AMA)
Sun, Huaibin& Tian, Jun& Xian, Wanhua& Xie, Tingting& Yang, Xiangdong. miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061074
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061074