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Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma
Joint Authors
Li, Yi
Zhang, Liqun
Liu, Fei
Xiang, Guiming
Jiang, Dongneng
Pu, Xiaoyun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Serum exosomal microRNAs (miRNAs) have received considerable attention as potential biomarkers for diagnosing cancer.
The canonical technique for measuring miRNA transcript levels is reverse transcription quantitative polymerase chain reaction (RT-qPCR).
One prerequisite for validating RT-qPCR data is proper normalization with respect to stably expressed endogenous reference genes.
However, genes that meet all of the criteria of a control gene for exosomal miRNAs have not yet been identified.
To find out the control gene for exosomal miRNAs, we evaluated the expression stability of 11 well-known reference genes in circulating exosomes.
In this study, we found that the combination of miR-221, miR-191, let-7a, miR-181a, and miR-26a can be an optimal gene reference set for normalizing the expression of liver-specific miRNAs.
This combination enhanced the robustness of the relative quantification analyses.
These findings highlight the importance of validating reference genes before quantifying target miRNAs.
Furthermore, our findings will improve studies that monitor hepatitis progression and will aid in the discovery of noninvasive biomarkers to diagnose early stage HCC.
American Psychological Association (APA)
Li, Yi& Zhang, Liqun& Liu, Fei& Xiang, Guiming& Jiang, Dongneng& Pu, Xiaoyun. 2015. Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma. Disease Markers،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061075
Modern Language Association (MLA)
Li, Yi…[et al.]. Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma. Disease Markers No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1061075
American Medical Association (AMA)
Li, Yi& Zhang, Liqun& Liu, Fei& Xiang, Guiming& Jiang, Dongneng& Pu, Xiaoyun. Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061075
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061075