Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways
Joint Authors
Hinkelbein, Jochen
Böhm, Lennert
Spelten, Oliver
Sander, David
Soltész, Stefan
Braunecker, Stefan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Introduction.
In renal tissue as well as in other organs, supranormal oxygen pressure may lead to deleterious consequences on a cellular level.
Additionally, hyperoxia-induced effect in cells and related free radicals may potentially contribute to renal failure.
The aim of this study was to analyze time-dependent alterations of rat kidney protein expression after short-term normobaric hyperoxia using proteomics and bioinformatic approaches.
Material and Methods.
N = 36 Wistar rats were randomized into six different groups: three groups with normobaric hyperoxia (exposure to 100% oxygen for 3 h) and three groups with normobaric normoxia (NN; room air).
After hyperoxia exposure, kidneys were removed immediately, after 3 days and after 7 days.
Kidney lysates were analyzed by two-dimensional gel electrophoresis followed by peptide mass fingerprinting using tandem mass spectrometry.
Statistical analysis was performed with DeCyder 2D software ( p < 0.01 ).
Biological functions of differential regulated proteins were studied using functional network analysis (Ingenuity Pathways Analysis and PathwayStudio).
Results.
Expression of 14 proteins was significantly altered ( p < 0.01 ) : eight proteins (MEP1A_RAT, RSSA_RAT, F16P1_RAT, STML2_RAT, BPNT1_RAT, LGMN_RAT, ATPA_RAT, and VDAC1_RAT) were downregulated and six proteins (MTUS1_RAT, F16P1_RAT, ACTG_RAT, ACTB_RAT, 2ABA_RAT, and RAB1A_RAT) were upregulated.
Bioinformatic analyses revealed an association of regulated proteins with inflammation.
Conclusions.
Significant alterations in renal protein expression could be demonstrated for up to 7 days even after short-term hyperoxia.
The identified proteins indicate an association with inflammation signaling cascades.
MEP1A and VDAC1 could be promising candidates to identify hyperoxic injury in kidney cells.
American Psychological Association (APA)
Hinkelbein, Jochen& Böhm, Lennert& Spelten, Oliver& Sander, David& Soltész, Stefan& Braunecker, Stefan. 2015. Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways. Disease Markers،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061089
Modern Language Association (MLA)
Hinkelbein, Jochen…[et al.]. Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways. Disease Markers No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1061089
American Medical Association (AMA)
Hinkelbein, Jochen& Böhm, Lennert& Spelten, Oliver& Sander, David& Soltész, Stefan& Braunecker, Stefan. Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061089
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061089