Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications
Joint Authors
Egidi, Maria Giulia
Cochetti, Giovanni
Guelfi, Gabriella
Zampini, Danilo
Mearini, Ettore
Diverio, Silvana
Poli, Giulia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We aimed at assessing the stability of candidate reference genes in urine sediments of men subjected to digital rectal examination for suspected prostate cancer (PCa).
Two microRNAs (miR-191 and miR-25) and 1 small nucleolar RNA (SNORD48) were assayed in 35 post-DRE urine sediments of men with PCa and in 26 subjects with histologically confirmed benign prostatic hyperplasia (BPH).
The stability of candidate reference genes was assessed through BestKeeper algorithm and equivalence test.
miR-200b and miR-452 were used to test for the effect of normalization on target genes.
Our results proved miR-191 to be the most stable gene, showing the lowest degree of variation and the highest stability value.
miR-25 and SNORD48 values fell beyond the cutoff of acceptability.
In conclusion, we recommend the use of miR-191 for normalization purposes in post-DRE urine sediments.
American Psychological Association (APA)
Egidi, Maria Giulia& Cochetti, Giovanni& Guelfi, Gabriella& Zampini, Danilo& Diverio, Silvana& Poli, Giulia…[et al.]. 2015. Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications. Disease Markers،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061090
Modern Language Association (MLA)
Egidi, Maria Giulia…[et al.]. Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications. Disease Markers No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1061090
American Medical Association (AMA)
Egidi, Maria Giulia& Cochetti, Giovanni& Guelfi, Gabriella& Zampini, Danilo& Diverio, Silvana& Poli, Giulia…[et al.]. Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061090
