Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy
Joint Authors
Zhu, Hao
Jiang, Chun-Bo
Wei, Ming-Gang
Tu, Yue
Li, Chun-Qing
Jing, Wei-Min
Sun, Wei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objectives.
We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury.
Methods.
In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group).
On days 7, 28, 42, and 56, 24 h urine samples were collected.
All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters.
Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels.
Results.
Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions.
Triptolide also elevated the nephrin protein level.
Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs.
Conclusions.
These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p.
American Psychological Association (APA)
Jiang, Chun-Bo& Wei, Ming-Gang& Tu, Yue& Zhu, Hao& Li, Chun-Qing& Jing, Wei-Min…[et al.]. 2015. Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1061145
Modern Language Association (MLA)
Jiang, Chun-Bo…[et al.]. Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1061145
American Medical Association (AMA)
Jiang, Chun-Bo& Wei, Ming-Gang& Tu, Yue& Zhu, Hao& Li, Chun-Qing& Jing, Wei-Min…[et al.]. Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1061145
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061145