Effect of Nourishing “Yin” Removing “Fire” Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats
Joint Authors
Yu, Jian
Tian, Zhanzhuang
Zeng, Gulan
Han, Xinghui
Wang, Yonghong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
The present study aims to investigate the effects of nourishing “Yin” removing “Fire” (NYRF) Chinese herbal mixture on puberty onset and hypothalamic mTOR expression in female rats.
Materials and Methods.
Forty female 20-day-old Sprague-Dawley rats were randomly divided into Chinese herbal mixture (CHM) and normal saline (NS) groups.
Rats in CHM and NS were treated with NYRF mixture and normal saline, respectively, from d22.
Rats in each group were sacrificed on d28, d31, and d34.
Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and estradiol (E2) levels were analyzed by ELISA.
Hypothalamic mTOR mRNA expression levels were determined by RT-PCR and hypothalamic p-mTOR protein levels were assayed by western blot.
Results.
The vaginal opening time in CHM group was significantly delayed P < 0.05 .
On d31, in comparison with NS group, the coefficients of uteri and ovaries, levels of serum LH and E2, and the expression levels of hypothalamic mTOR mRNA and p-mTOR protein were significantly lower in CHM group P < 0.05 .
Conclusion.
The mechanism by which the nourishing “Yin” removing “Fire” Chinese herbal mixture delays puberty onset may be associated with the inhibition of the hypothalamic mTOR signaling.
American Psychological Association (APA)
Zeng, Gulan& Han, Xinghui& Yu, Jian& Wang, Yonghong& Tian, Zhanzhuang. 2015. Effect of Nourishing “Yin” Removing “Fire” Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061185
Modern Language Association (MLA)
Zeng, Gulan…[et al.]. Effect of Nourishing “Yin” Removing “Fire” Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061185
American Medical Association (AMA)
Zeng, Gulan& Han, Xinghui& Yu, Jian& Wang, Yonghong& Tian, Zhanzhuang. Effect of Nourishing “Yin” Removing “Fire” Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061185
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061185