In Vitro Antibacterial, Antifungal, Antibiofilm, Antioxidant, and Anticancer Properties of Isosteviol Isolated from Endangered Medicinal Plant Pittosporum tetraspermum
Joint Authors
Valan Arasu, Mariadhas
Abdullah Al-Dhabi, Naif
Rejiniemon, Thankappan Sarasam
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study aimed to investigate the in vitro antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of isosteviol isolated from endangered medicinal plant Pittosporum tetraspermum.
Pure compound was obtained and characterized by column chromatography followed by 1H NMR, 13C NMR, IR, and mass spectral analysis.
The antimicrobial activities of the compound were assessed by the broth microdilution method and the antioxidant properties were determined using reducing ability assay, DPPH scavenging assay, hydroxyl radical scavenging activity, and superoxide radical scavenging assay.
Anticancer study was evaluated by following MTT assay.
Column purification and spectrocopical analysis lead to identifying isosteviol from the crude ethyl acetate extract.
The compound exhibited significant activity against bacteria such as Staphylococcus epidermidis (125 µg/mL), Staphylococcus aureus (125 µg/mL), and Klebsiella pneumoniae (62.5 µg/mL).
The MIC of the compound against Candida albicans, Aspergillus niger, and Trichophyton mentagrophytes was 62.5, 125, and 500 µg/mL, respectively.
The compound showed comparatively better antibiofilm activity against E.
coli, S.
typhi, and P.
aeruginosa.
Furthermore, it exhibited good antioxidant properties.
Anticancer properties of the compound against Vero and MCF7 cell lines were its advantage.
Novel isosteviol would be useful to reduce the infectious diseases caused by pathogenic microorganisms or slow the progress of various oxidative stress-related diseases.
American Psychological Association (APA)
Abdullah Al-Dhabi, Naif& Valan Arasu, Mariadhas& Rejiniemon, Thankappan Sarasam. 2015. In Vitro Antibacterial, Antifungal, Antibiofilm, Antioxidant, and Anticancer Properties of Isosteviol Isolated from Endangered Medicinal Plant Pittosporum tetraspermum. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1061195
Modern Language Association (MLA)
Abdullah Al-Dhabi, Naif…[et al.]. In Vitro Antibacterial, Antifungal, Antibiofilm, Antioxidant, and Anticancer Properties of Isosteviol Isolated from Endangered Medicinal Plant Pittosporum tetraspermum. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1061195
American Medical Association (AMA)
Abdullah Al-Dhabi, Naif& Valan Arasu, Mariadhas& Rejiniemon, Thankappan Sarasam. In Vitro Antibacterial, Antifungal, Antibiofilm, Antioxidant, and Anticancer Properties of Isosteviol Isolated from Endangered Medicinal Plant Pittosporum tetraspermum. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1061195
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061195