Huaier Aqueous Extract Induces Hepatocellular Carcinoma Cells Arrest in S Phase via JNK Signaling Pathway
Joint Authors
Zhang, Chengshuo
Zhang, Jialin
Li, Xin
Sun, Ning
Yu, Rui
Zhao, Bochao
Yu, Dongyang
Cheng, Ying
Liu, Yongfeng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Huaier aqueous extract, the main active constituent of Huaier proteoglycan, has antihepatocarcinoma activity in experimental and clinical settings.
However, the potential and associated antihepatoma mechanisms of Huaier extract are not yet fully understood.
Therefore, in this study, we aimed to elucidate the inhibitory proliferation effect of Huaier extract on apoptosis and cycle of HepG2 and Bel-7402 cells.
Our data demonstrated that incubation with Huaier extract resulted in a marked decrease in cell viability dose-dependently.
Flow cytometric analysis showed that a 48 h treatment of Huaier extract caused cell apoptosis.
Typical apoptotic nucleus alterations were observed with fluorescence microscope after Hoechst staining.
Immunoblot analysis further demonstrated that Huaier extract activated caspase 3 and PARP.
Additionally, Huaier extract inhibited the activity of p-ERK, p-p38, and p-JNK in terms of MAPK.
Furthermore, Huaier extract induced HCC cells arrest in S phase and decreased the cycle related protein expression of β-catenin and cyclin D1.
Studies with JNK specific inhibitor, SP600125, showed that Huaier extract induced S phase arrest and decreased β-catenin and cyclin D1 expression via JNK signaling pathway.
In conclusion, we verify that Huaier extract causes cell apoptosis and induces hepatocellular carcinoma cells arrest in S phase via JNK pathway, which advances our understanding on the molecular mechanisms of Huaier extract in hepatocarcinoma management.
American Psychological Association (APA)
Zhang, Chengshuo& Zhang, Jialin& Li, Xin& Sun, Ning& Yu, Rui& Zhao, Bochao…[et al.]. 2015. Huaier Aqueous Extract Induces Hepatocellular Carcinoma Cells Arrest in S Phase via JNK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1061203
Modern Language Association (MLA)
Zhang, Chengshuo…[et al.]. Huaier Aqueous Extract Induces Hepatocellular Carcinoma Cells Arrest in S Phase via JNK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1061203
American Medical Association (AMA)
Zhang, Chengshuo& Zhang, Jialin& Li, Xin& Sun, Ning& Yu, Rui& Zhao, Bochao…[et al.]. Huaier Aqueous Extract Induces Hepatocellular Carcinoma Cells Arrest in S Phase via JNK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1061203
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061203