![](/images/graphics-bg.png)
Optimization on Preparation Conditions of Salidroside Liposome and Its Immunological Activity on PCV-2 in Mice
Joint Authors
Feng, Yibo
Zhao, Xiaojuan
Lv, Fang
Zhang, Jinqiu
Deng, Bihua
Zhao, Yanhong
Lu, Yu
Bo, Ruonan
Liu, Zhenguang
Liu, Jiaguo
Wang, Deyun
Hu, Yuanliang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to optimize the preparation conditions of salidroside liposome with high encapsulation efficiency (EE) and to study the immunological enhancement activity of salidroside liposome as porcine circovirus type 2 virus (PCV-2) vaccine adjuvant.
Response surface methodology (RSM) was selected to optimize the conditions for the preparation of salidroside liposome using Design-Expert V8.0.6 software.
Three kinds of salidroside liposome adjuvants were prepared to study their adjuvant activity.
BALB/c mice were immunized with PCV-2 encapsulated in different kinds of salidroside liposome adjuvants.
The PCV-2-specific IgG in immunized mice serum was determined with ELISA.
The results showed that when the concentration of ammonium sulfate was 0.26 mol·L−1, ethanol volume 6.5 mL, temperature 43°C, ethanol injection rate 3 mL·min−1, and salidroside liposome could be prepared with high encapsulation efficiency of 94.527%.
Salidroside liposome as adjuvant could rapidly induce the production of PCV-2-specific IgG and salidroside liposome I adjuvant proved to provide the best effect among the three kinds of salidroside liposome adjuvants.
American Psychological Association (APA)
Feng, Yibo& Zhao, Xiaojuan& Lv, Fang& Zhang, Jinqiu& Deng, Bihua& Zhao, Yanhong…[et al.]. 2015. Optimization on Preparation Conditions of Salidroside Liposome and Its Immunological Activity on PCV-2 in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061209
Modern Language Association (MLA)
Feng, Yibo…[et al.]. Optimization on Preparation Conditions of Salidroside Liposome and Its Immunological Activity on PCV-2 in Mice. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1061209
American Medical Association (AMA)
Feng, Yibo& Zhao, Xiaojuan& Lv, Fang& Zhang, Jinqiu& Deng, Bihua& Zhao, Yanhong…[et al.]. Optimization on Preparation Conditions of Salidroside Liposome and Its Immunological Activity on PCV-2 in Mice. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061209
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061209