The Impact of Paeoniflorin on α-Synuclein Degradation Pathway
Joint Authors
Cai, Zenglin
Zhang, Xinzhi
Zhang, Yongjin
Li, Xiuming
Xu, Jing
Li, Xiaomin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Paeoniflorin (PF) is the major active ingredient in the traditional Chinese medicine Radix.
It plays a neuroprotective role by regulating autophagy and the ubiquitin-proteasome degradation pathway.
In this study, we found PF significantly reduced cell damage caused by MPP+, returning cells to normal state.
Cell viability significantly improved after 24 h exposure to RAPA and PF in the MPP+ group (all P<0.01).
CAT and SOD activities were significantly decreased after PF and RAPA treatment, compared with MPP+ (P<0.001).
In addition, MPP+ activated both LC3-II and E1; RAPA increased LC3-II but inhibited E1.
PF significantly upregulated both LC3-II (autophagy) and E1 (ubiquitin-proteasome pathway) expression (P<0.001), promoted degradation of α-synuclein, and reduced cell damage.
We show MPP+ enhanced immunofluorescence signal of intracellular α-synuclein and LC3.
Fluorescence intensity of α-synuclein decreased after PF treatment.
In conclusion, these data show PF reversed the decline of proteasome activity caused by MPP+ and significantly upregulated both autophagy and ubiquitin-proteasome pathways, promoted the degradation of α-synuclein, and reduced cell damage.
These findings suggest PF is a potential therapeutic medicine for neurodegenerative diseases.
American Psychological Association (APA)
Cai, Zenglin& Zhang, Xinzhi& Zhang, Yongjin& Li, Xiuming& Xu, Jing& Li, Xiaomin. 2015. The Impact of Paeoniflorin on α-Synuclein Degradation Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061214
Modern Language Association (MLA)
Cai, Zenglin…[et al.]. The Impact of Paeoniflorin on α-Synuclein Degradation Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061214
American Medical Association (AMA)
Cai, Zenglin& Zhang, Xinzhi& Zhang, Yongjin& Li, Xiuming& Xu, Jing& Li, Xiaomin. The Impact of Paeoniflorin on α-Synuclein Degradation Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061214