The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3KAkt Pathway in Human Renal Proximal Tubular Epithelial Cells
Joint Authors
Pae, Hyun-Ock
Ju, Sung Min
Kang, Jun Gue
Bae, Jun Sang
Lyu, Yeoung Su
Jeon, Byung Hun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Apigenin is a member of the flavone subclass of flavonoids present in fruits and vegetables.
Apigenin has long been considered to have various biological activities, such as antioxidant, anti-inflammatory, and antitumorigenic properties, in various cell types.
Cisplatin was known to exhibit cytotoxic effect to renal cells by inducing apoptosis through activation of p53.
The present study investigated the antiapoptotic effects of apigenin on the cisplatin-treated human renal proximal tubular epithelial (HK-2) cells.
HK-2 cells were pretreated with apigenin (5, 10, 20 μM) for 1 h and then treated with 40 μM cisplatin for various times.
Apigenin inhibited the cisplatin-induced apoptosis of HK-2 cells.
Interestingly, apigenin itself exerted cytostatic activity because of its ability to induce cell cycle arrest.
Apigenin inhibited caspase-3 activity and PARP cleavage in cisplatin-treated cells.
Apigenin reduced cisplatin-induced phosphorylation and expression of p53, with no significant influence on production of ROS that is known to induce p53 activation.
Furthermore, apigenin promoted cisplatin-induced Akt phosphorylation, suggesting that enhanced Akt activation may be involved in cytoprotection.
Taken together, these results suggest that apigenin ameliorates cisplatin-induced apoptosis through reduction of p53 activation and promotion of PI3K/Akt pathway in HK-2 cells.
American Psychological Association (APA)
Ju, Sung Min& Kang, Jun Gue& Bae, Jun Sang& Pae, Hyun-Ock& Lyu, Yeoung Su& Jeon, Byung Hun. 2015. The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3KAkt Pathway in Human Renal Proximal Tubular Epithelial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061218
Modern Language Association (MLA)
Ju, Sung Min…[et al.]. The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3KAkt Pathway in Human Renal Proximal Tubular Epithelial Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1061218
American Medical Association (AMA)
Ju, Sung Min& Kang, Jun Gue& Bae, Jun Sang& Pae, Hyun-Ock& Lyu, Yeoung Su& Jeon, Byung Hun. The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3KAkt Pathway in Human Renal Proximal Tubular Epithelial Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061218