The Activation of Nrf2 and Its Downstream Regulated Genes Mediates the Antioxidative Activities of Xueshuan Xinmaining Tablet in Human Umbilical Vein Endothelial Cells
Joint Authors
Xiong, Lingxin
Xie, Jingshu
Song, Chenxue
Liu, Jinping
Zheng, Jingtong
Liu, Chuangui
Zhang, Xiaotian
Li, Pingya
Wang, Fang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Epidemiological studies have verified the critical role that antioxidative stress plays in protecting vascular endothelial cells.
The aims of the present study were to investigate the antioxidative activities and differential regulation of nuclear erythroid-related factor 2- (Nrf2-) mediated gene expression by Xueshuan Xinmaining Tablet (XXT), a traditional Chinese medicine with the effect of treating cardiovascular diseases.
The antioxidative activities of XXT were investigated using quantitative real-time PCR (qPCR), a PCR array, and western blotting.
Our results indicated that XXT exhibited potent antioxidative activities by suppressing the levels of hydrogen peroxide- (H2O2-) induced reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs).
We were also conscious of strong Nrf2-mediated antioxidant induction.
XXT enhanced the expressions of Keap1, Nrf2, and Nrf2-mediated genes, such as glutamate-cysteine ligase modifier subunit (GCLM), NAD(P)H: quinine oxidoreductase 1 (NQO1), heme oxygenase 1 (HMOX1), and glutathione peroxidase (GPX) in HUVECs.
In summary, XXT strongly activated Nrf2 and its downstream regulated genes, which may contribute to the antioxidative and vascular endothelial cell protective activities of XXT.
American Psychological Association (APA)
Xiong, Lingxin& Xie, Jingshu& Song, Chenxue& Liu, Jinping& Zheng, Jingtong& Liu, Chuangui…[et al.]. 2015. The Activation of Nrf2 and Its Downstream Regulated Genes Mediates the Antioxidative Activities of Xueshuan Xinmaining Tablet in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1061219
Modern Language Association (MLA)
Xiong, Lingxin…[et al.]. The Activation of Nrf2 and Its Downstream Regulated Genes Mediates the Antioxidative Activities of Xueshuan Xinmaining Tablet in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1061219
American Medical Association (AMA)
Xiong, Lingxin& Xie, Jingshu& Song, Chenxue& Liu, Jinping& Zheng, Jingtong& Liu, Chuangui…[et al.]. The Activation of Nrf2 and Its Downstream Regulated Genes Mediates the Antioxidative Activities of Xueshuan Xinmaining Tablet in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1061219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061219