Sedum mexicanum Britt. Induces Apoptosis of Primary Rat Activated Hepatic Stellate Cells
Joint Authors
Lee, Shou-Lun
Chin, Ting-Yu
Lai, Ching-Long
Wang, Wen-Han
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Liver fibrosis is a significant liver disease in Asian countries.
Sedum mexicanum Britt.
(SM) has been claimed to have antihepatitis efficacy.
In traditional folk medicine, a solution of boiling water-extracted SM (SME) is consumed to prevent and treat hepatitis.
However, its efficacy has not yet been verified.
The purpose of this study was to investigate the in vitro effect of SME on hepatoprotection.
Methods.
Hepatic stellate cells (HSCs) and hepatocytes (HCs) were isolated from the livers of the rats by enzymatic digestion and density gradient centrifugation.
Results.
Treating the HCs and aHSCs with SME caused a dose-dependent decrease in the viability of aHSCs but not that of HCs.
In addition, treatment with SME resulted in apoptosis of aHSCs, as determined by DAPI analysis and flow cytometry.
SME also increased the amount of cleaved caspase-3, cleaved caspase-9, and cleaved poly ADP-ribose polymerase (PARP) in aHSCs.
Furthermore, SME treatment induced a dose-dependent reduction in Bcl-2 expression and increased the expression of Bax in aHSCs.
Conclusions.
SME did not cause cytotoxicity in HCs, but it induced apoptosis in aHSCs through the mitochondria-dependent caspase-3 pathway.
Therefore, SME may possess therapeutic potential for liver fibrosis.
American Psychological Association (APA)
Lee, Shou-Lun& Chin, Ting-Yu& Lai, Ching-Long& Wang, Wen-Han. 2015. Sedum mexicanum Britt. Induces Apoptosis of Primary Rat Activated Hepatic Stellate Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061229
Modern Language Association (MLA)
Lee, Shou-Lun…[et al.]. Sedum mexicanum Britt. Induces Apoptosis of Primary Rat Activated Hepatic Stellate Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061229
American Medical Association (AMA)
Lee, Shou-Lun& Chin, Ting-Yu& Lai, Ching-Long& Wang, Wen-Han. Sedum mexicanum Britt. Induces Apoptosis of Primary Rat Activated Hepatic Stellate Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061229