Antrodia camphorata Potentiates Neuroprotection against Cerebral Ischemia in Rats via Downregulation of iNOSHO-1Bax and Activated Caspase-3 and Inhibition of Hydroxyl Radical Formation
Joint Authors
Yen, Ting-Lin
Lan, Chang-Chou
Yang, Po-Sheng
Lin, Po-Yen
Yu, Meng-Che
Yang, Chih-Hao
Chang, Chao-Chien
Jayakumar, Thanasekaran
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Antrodia camphorata (A.
camphorata) is a fungus generally used in Chinese folk medicine for treatment of viral hepatitis and cancer.
Our previous study found A.
camphorata has neuroprotective properties and could reduce stroke injury in cerebral ischemia animal models.
In this study, we sought to investigate the molecular mechanisms of neuroprotective effects of A.
camphorata in middle cerebral artery occlusion (MCAO) rats.
A selective occlusion of the middle cerebral artery (MCA) with whole blood clots was used to induce ischemic stroke in rats and they were orally treated with A.
camphorata (0.25 and 0.75 g/kg/day) alone or combined with aspirin (5 mg/kg/day).
To provide insight into the functions of A.
camphorata mediated neuroprotection, the expression of Bax, inducible nitric oxide synthase (iNOS), haem oxygenase-1 (HO-1), and activated caspase-3 was determined by Western blot assay.
Treatment of aspirin alone significantly reduced the expressions of HO-1 (P<0.001), iNOS (P<0.001), and Bax (P<0.01) in ischemic regions.
The reduction of these expressions was more potentiated when rats treated by aspirin combined with A.
camphorata (0.75 g/kg/day).
Combination treatment also reduced apoptosis as measured by a significant reduction in active caspase-3 expression in the ischemic brain compared to MCAO group (P<0.01).
Moreover, treatment of A.
camphorata significantly (P<0.05) reduced fenton reaction-induced hydroxyl radical (OH•) formation at a dose of 40 mg/mL.
Taken together, A.
camphorata has shown neuroprotective effects in embolic rats, and the molecular mechanisms may correlate with the downregulation of Bax, iNOS, HO-1, and activated caspase-3 and the inhibition of OH• signals.
American Psychological Association (APA)
Yang, Po-Sheng& Lin, Po-Yen& Chang, Chao-Chien& Yu, Meng-Che& Yen, Ting-Lin& Lan, Chang-Chou…[et al.]. 2015. Antrodia camphorata Potentiates Neuroprotection against Cerebral Ischemia in Rats via Downregulation of iNOSHO-1Bax and Activated Caspase-3 and Inhibition of Hydroxyl Radical Formation. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061260
Modern Language Association (MLA)
Yang, Po-Sheng…[et al.]. Antrodia camphorata Potentiates Neuroprotection against Cerebral Ischemia in Rats via Downregulation of iNOSHO-1Bax and Activated Caspase-3 and Inhibition of Hydroxyl Radical Formation. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061260
American Medical Association (AMA)
Yang, Po-Sheng& Lin, Po-Yen& Chang, Chao-Chien& Yu, Meng-Che& Yen, Ting-Lin& Lan, Chang-Chou…[et al.]. Antrodia camphorata Potentiates Neuroprotection against Cerebral Ischemia in Rats via Downregulation of iNOSHO-1Bax and Activated Caspase-3 and Inhibition of Hydroxyl Radical Formation. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061260
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061260