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Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is Dependent on Protein-Nutrient Concentration
Joint Authors
Yen, Feng-Lin
Lee, Chiang-Wen
Hu, Stephen Chu-Sung
Lin, Chi-Ling
Cheng, Hui-Min
Chen, Gwo-Shing
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Artocarpin, a natural prenylated flavonoid, has been shown to have various biological properties.
However, its effects on human cutaneous squamous cell carcinoma (SCC) have not been previously investigated.
We set out to determine whether artocarpin has cytotoxic effects on SCC cells and whether its pharmacological activity is dependent on protein-nutrient concentration.
Our results showed that treatment of HSC-1 cells (a human cutaneous SCC cell line) with artocarpin decreased cell viability and induced cell apoptosis by increasing caspase 3/7 activity.
These effects were more pronounced at low fetal bovine serum (FBS) concentrations.
Artocarpin induced an increase in the level of phospho-p38 and a decrease in the levels of phospho-ERK, phospho-JNK, phospho-Akt, phospho-mTOR, and phospho-S6K.
High FBS concentrations in the culture media inhibited and delayed the uptake of artocarpin from the extracellular compartment (culture media) into the intracellular compartment, as determined by high performance liquid chromatography (HPLC) analysis.
In conclusion, artocarpin induces apoptosis in HSC-1 cells through modulation of MAPK and Akt/mTOR pathways.
Binding of artocarpin to proteins in the FBS may inhibit cellular uptake and reduce the cytotoxic activity of artocarpin on HSC-1 cells.
Therefore, artocarpin may have potential use in the future as a form of treatment for cutaneous SCC.
American Psychological Association (APA)
Hu, Stephen Chu-Sung& Lin, Chi-Ling& Cheng, Hui-Min& Chen, Gwo-Shing& Lee, Chiang-Wen& Yen, Feng-Lin. 2015. Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is Dependent on Protein-Nutrient Concentration. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061263
Modern Language Association (MLA)
Hu, Stephen Chu-Sung…[et al.]. Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is Dependent on Protein-Nutrient Concentration. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061263
American Medical Association (AMA)
Hu, Stephen Chu-Sung& Lin, Chi-Ling& Cheng, Hui-Min& Chen, Gwo-Shing& Lee, Chiang-Wen& Yen, Feng-Lin. Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is Dependent on Protein-Nutrient Concentration. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061263
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061263