Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0G1-Phase Cell Cycle Arrest and Apoptosis
Joint Authors
Xi, Song-yang
Teng, Yu-hao
Chen, Yan
Li, Jie-ping
Zhang, Ying-ying
Liu, Shen-lin
Zou, Xi
Zhou, Jin-yong
Wu, Jian
Wang, Ruiping
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Jianpi Huayu Decoction (JHD), a Chinese medicine formula, is a typical prescription against multiple tumors in the clinical treatment, which can raise quality of life and decrease complications.
The aim of this study is to assess the efficacy of JHD against human colorectal carcinoma cells (SW480) and explore its mechanism.
MTT assay showed that JHD decreased the cellular viability of SW480 cells in dose-dependent and time-dependent manner.
Flow cytometry analysis revealed that JHD induced G0/G1-phase cell cycle arrest in SW480 cells and had a strong apoptosis-inducing effect on SW480 cells.
Meanwhile it enhanced the expression of p27, cleaved PARP, cleaved caspase-3, and Bax and decreased the levels of PARP, caspase-3, Bcl-2, CDK2, CDK4, CDK6, cyclin D1, cyclin D2, cyclin D3, and cyclin E1, which was evidenced by RT-qPCR and Western blot analysis.
In conclusion, these results indicated that JHD inhibited proliferation in SW480 cells by inducing G0/G1-phase cell cycle arrest and apoptosis, providing a practicaltherapeutic strategy against colorectal cancer.
American Psychological Association (APA)
Xi, Song-yang& Teng, Yu-hao& Chen, Yan& Li, Jie-ping& Zhang, Ying-ying& Liu, Shen-lin…[et al.]. 2015. Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0G1-Phase Cell Cycle Arrest and Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061265
Modern Language Association (MLA)
Xi, Song-yang…[et al.]. Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0G1-Phase Cell Cycle Arrest and Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061265
American Medical Association (AMA)
Xi, Song-yang& Teng, Yu-hao& Chen, Yan& Li, Jie-ping& Zhang, Ying-ying& Liu, Shen-lin…[et al.]. Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0G1-Phase Cell Cycle Arrest and Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061265