Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells
Joint Authors
Šmejkal, Karel
Kollar, Peter
Závalová, Veronika Müller
Keltošová, Stanislava
Trnová, Pavlína
Fedr, Radek
Bárta, Tomáš
Souček, Karel
Hošek, Jan
Hampl, Aleš
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aims.
In this work we studied cytodifferentiation effects of newly characterized prenyl flavonoid 4′-O-methylkuwanon E (4ME) isolated from white mulberry (Morus alba L.).
Main Methods.
Cell growth and viability were measured by dye exclusion assay; cell cycle and surface antigen CD11b were monitored by flow cytometry.
For the cytodifferentiation of cells the NBT reduction assay was employed.
Regulatory proteins were assessed by western blotting.
Key Findings.
4ME induced dose-dependent growth inhibition of THP-1 cells, which was not accompanied by toxic effect.
Inhibition of cells proliferation caused by 4ME was associated with the accumulation in G1 phase and with downregulation of hyperphosphorylated pRb.
Treatment with 4ME led to significant induction of NBT-reducing activity of PMA stimulated THP-1 cells and upregulation expression of differentiation-associated surface antigen CD11b.
Our results suggest that monocytic differentiation induced by 4ME is connected with up-regulation of p38 kinase activity.
Significance.
Our study provides the first evidence that 4ME induces the differentiation of THP-1 human monocytic leukemia cells and thus is a potential cytodifferentiating anticancer agent.
American Psychological Association (APA)
Kollar, Peter& Bárta, Tomáš& Keltošová, Stanislava& Trnová, Pavlína& Závalová, Veronika Müller& Šmejkal, Karel…[et al.]. 2015. Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061277
Modern Language Association (MLA)
Kollar, Peter…[et al.]. Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061277
American Medical Association (AMA)
Kollar, Peter& Bárta, Tomáš& Keltošová, Stanislava& Trnová, Pavlína& Závalová, Veronika Müller& Šmejkal, Karel…[et al.]. Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061277
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061277