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Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway
Joint Authors
Huang, Wen-Chung
Liou, Chian-Jiun
Lai, Xuan-Yu
Chen, Ya-Ling
Wang, Chia-Ling
Wei, Ciao-Han
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ginkgolide C, isolated from Ginkgo biloba leaves, is a flavone reported to have multiple biological functions, from decreased platelet aggregation to ameliorating Alzheimer disease.
The study aim was to evaluate the antiadipogenic effect of ginkgolide C in 3T3-L1 adipocytes.
Ginkgolide C was used to treat differentiated 3T3-L1 cells.
Cell supernatant was collected to assay glycerol release, and cells were lysed to measure protein and gene expression related to adipogenesis and lipolysis by western blot and real-time PCR, respectively.
Ginkgolide C significantly suppressed lipid accumulation in differentiated adipocytes.
It also decreased adipogenesis-related transcription factor expression, including peroxisome proliferator-activated receptor and CCAAT/enhancer-binding protein.
Furthermore, ginkgolide C enhanced adipose triglyceride lipase and hormone-sensitive lipase production for lipolysis and increased phosphorylation of AMP-activated protein kinase (AMPK), resulting in decreased activity of acetyl-CoA carboxylase for fatty acid synthesis.
In coculture with an AMPK inhibitor (compound C), ginkgolide C also improved activation of sirtuin 1 and phosphorylation of AMPK in differentiated 3T3-L1 cells.
The results suggest that ginkgolide C is an effective flavone for increasing lipolysis and inhibiting adipogenesis in adipocytes through the activated AMPK pathway.
American Psychological Association (APA)
Liou, Chian-Jiun& Lai, Xuan-Yu& Chen, Ya-Ling& Wang, Chia-Ling& Wei, Ciao-Han& Huang, Wen-Chung. 2015. Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061331
Modern Language Association (MLA)
Liou, Chian-Jiun…[et al.]. Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1061331
American Medical Association (AMA)
Liou, Chian-Jiun& Lai, Xuan-Yu& Chen, Ya-Ling& Wang, Chia-Ling& Wei, Ciao-Han& Huang, Wen-Chung. Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061331
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061331