![](/images/graphics-bg.png)
Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds
Joint Authors
Mohamed, Saleh A.
Elshal, Mohamed F.
Kumosani, Taha A.
Aldahlawi, Alia M.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
L-asparaginase from bacteria has been used in treatment of acute lymphoblastic leukemia.
The aim of this study was to purify and characterize L-asparaginase from Phaseolus vulgaris seeds instead of microbial sources.
L-asparaginase was purified to apparent homogeneity.
The enzyme has molecular mass of 79 kDa.
The purified asparaginase had very low activity toward a number of asparagine and glutamine analogues.
L-asparaginase was free from glutaminase activity.
Kinetic parameters, Km and Vmax of purified enzyme, were found to be 6.72 mM and 0.16 μM, respectively.
The enzyme had optimum pH at 8.0.
The enzyme showed high stability at alkaline pH (pH 7.5–9.0) when incubated for up to 24 h.
L-asparaginase had the same temperature optimum and thermal stability at 37°C.
K+ was able to greatly enhance the activity of asparaginase by 150% compared with other metals tested.
In conclusion, L-asparaginase showed no glutaminase activity and good stability over a wide range of physiological conditions, and thus it could be used as a potential candidate for treatment of acute lymphoblastic leukemia.
American Psychological Association (APA)
Mohamed, Saleh A.& Elshal, Mohamed F.& Kumosani, Taha A.& Aldahlawi, Alia M.. 2015. Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061337
Modern Language Association (MLA)
Mohamed, Saleh A.…[et al.]. Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1061337
American Medical Association (AMA)
Mohamed, Saleh A.& Elshal, Mohamed F.& Kumosani, Taha A.& Aldahlawi, Alia M.. Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061337
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061337