Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa
Joint Authors
Moraes, Izabela Barbosa
Manzan-Martins, Camilla
de Gouveia, Neire Moura
Calábria, Luciana Karen
Hiraki, Karen Renata Nakamura
Moraes, Alberto da Silva
Espindola, Foued Salmen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Diabetes mellitus (DM) is characterized by hyperglycemia and alterations in the metabolism of lipids, carbohydrates, and proteins.
Due to its hypoglycemic effect Vochysia rufa is frequently used in Uberlandia, Brazil, to treat DM.
Despite its popularity, there is little information about its effect on hepatic tissue.
Therefore, we evaluated the histoarchitecture, oxidative stress parameters, and polyploidy of liver tissue from streptozotocin- (STZ-) induced diabetic rats treated with aqueous extract of Vochysia rufa (AEV).
Histology was determined by fixing the livers, processing, and staining with HE.
Oxidative stress was determined by evaluating CAT, GPx, and SOD activity in liver homogenates and hepatic mitochondria fraction and by measuring GST, GSH levels and lipid peroxidation (MDA).
Polyploidy was determined by subjecting isolated hepatocyte nuclei to flow cytometry.
In the diabetic group, GST activity and GSH rates decreased whereas liver homogenate analysis showed that GPx, SOD activity and MDA increased.
AEV treatment restored all parameters to normal levels.
The oxidative stress analysis of hepatic mitochondria fraction showed similar results.
Lower polyploid cell populations were found in the diabetic rat livers, even after glibenclamide treatment.
Thus, AEV treatment efficiently reduced hepatic oxidative stress caused by STZ-induced diabetes and produced no morphological changes in the histological analysis.
American Psychological Association (APA)
Moraes, Izabela Barbosa& Manzan-Martins, Camilla& de Gouveia, Neire Moura& Calábria, Luciana Karen& Hiraki, Karen Renata Nakamura& Moraes, Alberto da Silva…[et al.]. 2015. Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061345
Modern Language Association (MLA)
Moraes, Izabela Barbosa…[et al.]. Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1061345
American Medical Association (AMA)
Moraes, Izabela Barbosa& Manzan-Martins, Camilla& de Gouveia, Neire Moura& Calábria, Luciana Karen& Hiraki, Karen Renata Nakamura& Moraes, Alberto da Silva…[et al.]. Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1061345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061345