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miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia
Joint Authors
Shen, Aling
Hong, Zhenfeng
Liu, Liya
Wan, Yun
Zhao, Jinyan
Lin, Jiumao
Zhong, Xiaoyong
Zhang, Yuchen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
The objective of this study was to evaluate the molecular mechanism by which Qianliening capsule (QC) treats benign prostatic hyperplasia (BPH).
Methods.
Benign prostatic hyperplasia epithelial cell line BPH-1 was treated with 0, 1.25, 2.5, and 5 mg/mL QC for 48 h, respectively.
Evaluation of cell viability and observation of morphologic changes of BPH-1 cell gene expression and miRNA expression profiles were analyzed.
Real-time quantitative PCR was used to confirm changes in miRNA and gene expression.
GO and KEGG pathway-based approaches were used to investigate biological functions and signaling pathways affected by differentially expressed mRNAs.
Results.
QC inhibited BPH-1 cell proliferation.
Differential expression of 19 upregulated and 2 downregulated miRNAs was observed in QC-treated BPH-1 cells compared to untreated control cells.
107 upregulated and 71 downregulated genes were identified between the two groups.
Significantly enriched signaling pathways based on deregulated mRNAs were mainly involved in regulation of cell proliferation, apoptosis, and so on.
Additionally, miRNA-mRNA network analysis integrated these miRNAs and genes by outlining interactions of miRNA and related genes.
Conclusion.
The study was the first report of differentially expressed miRNA and mRNA in QC-treated BPH-1 cells.
American Psychological Association (APA)
Liu, Liya& Wan, Yun& Shen, Aling& Zhao, Jinyan& Lin, Jiumao& Zhong, Xiaoyong…[et al.]. 2015. miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061409
Modern Language Association (MLA)
Liu, Liya…[et al.]. miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1061409
American Medical Association (AMA)
Liu, Liya& Wan, Yun& Shen, Aling& Zhao, Jinyan& Lin, Jiumao& Zhong, Xiaoyong…[et al.]. miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061409