Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt
Joint Authors
Kim, Bonglee
Kwon, Oh Sung
Jeong, Myoung Seok
Kim, Sung-Hoon
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Though dietary azuki bean (Vigna angularis) seed containing antioxidant proanthocyanidins was known to have multibiological activities including antioxidant, hypotensive, anti-inflammatory, and immunomodulatory activities, the antiangiogenic activity of ethanol extract of Vigna angularis (EVA) was never reported so far.
In the present study, the antiangiogenic mechanism of EVA was examined in human umbilical vein endothelial cells (HUVECs).
EVA showed weak cytotoxicity in HUVECs, while it significantly suppressed the VEGF induced proliferation of HUVECs.
Consistently, wound healing assay revealed that EVA inhibited the VEGF induced migration of HUVECs.
Also, EVA abrogated the VEGF induced tube formation of HUVECs in a concentration dependent fashion.
Furthermore, Matrigel plug assay showed that EVA significantly reduced the hemoglobin level of Matrigel plug in mice compared to untreated control.
Of note, EVA effectively attenuated the phosphorylation of VEGFR2, Erk, and Akt in VEGF-treated HUVECs.
Overall, our findings suggest that EVA inhibits angiogenesis in VEGF-treated HUVECs via inhibition of phosphorylation of VEGFR2, ERK, and Akt.
American Psychological Association (APA)
Kwon, Oh Sung& Jeong, Myoung Seok& Kim, Bonglee& Kim, Sung-Hoon. 2015. Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061421
Modern Language Association (MLA)
Kwon, Oh Sung…[et al.]. Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1061421
American Medical Association (AMA)
Kwon, Oh Sung& Jeong, Myoung Seok& Kim, Bonglee& Kim, Sung-Hoon. Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061421