Si Shen Wan Regulates Phospholipase C γ -1 and PI3KAkt Signal in Colonic Mucosa from Rats with Colitis
Joint Authors
Xu, Rong
Huang, Hong-yan
Wang, Xin
Zou, Yong
Yue, Hai-yang
Liu, Duan-Yong
Huang, Min-Fang
Zhao, Hai-Mei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The present study explored the feasible pathway of Si Shen Wan (SSW) in inhibiting apoptosis of intestinal epithelial cells (IECs) by observing activation of phospholipase Cγ-1 (PLC-γ1) and PI3K/Akt signal in colonic mucosa from rats with colitis.
Experimental colitis was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in the Sprague-Dawley rats.
After SSW was administrated for 7 days after TNBS infusion, western blot showed an increment in levels of PI3K, p-Akt, and IL-23 and a decrement in levels of PLC-γ1 and HSP70 in colonic mucosal injury induced by TNBS.
Meanwhile, assessments by ELISA revealed an increment in concentrations of IL-2, IL-6, and IL-17 and a reduction in level of TGF-β after TNBS challenge.
Impressively, treatment with SSW for 7 days significantly attenuated the expressions of PI3K and p-Akt and the secretion of IL-2, IL-6, IL-17, and IL-23 and promoted the activation of PLC-γ1, HSP70, and TGF-β.
Our previous studies had demonstrated that SSW restored colonic mucosal ulcers by inhibiting apoptosis of IECs.
The present study demonstrated that the effect of SSW on inhibiting apoptosis of IECs was realized probably by activation of PLC-γ1 and suppression of PI3K/Akt signal pathway.
American Psychological Association (APA)
Liu, Duan-Yong& Xu, Rong& Huang, Min-Fang& Huang, Hong-yan& Wang, Xin& Zou, Yong…[et al.]. 2015. Si Shen Wan Regulates Phospholipase C γ -1 and PI3KAkt Signal in Colonic Mucosa from Rats with Colitis. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1061448
Modern Language Association (MLA)
Liu, Duan-Yong…[et al.]. Si Shen Wan Regulates Phospholipase C γ -1 and PI3KAkt Signal in Colonic Mucosa from Rats with Colitis. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1061448
American Medical Association (AMA)
Liu, Duan-Yong& Xu, Rong& Huang, Min-Fang& Huang, Hong-yan& Wang, Xin& Zou, Yong…[et al.]. Si Shen Wan Regulates Phospholipase C γ -1 and PI3KAkt Signal in Colonic Mucosa from Rats with Colitis. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1061448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061448