CDRI-08 Attenuates RESTNRSF-Mediated Expression of NMDAR1 Gene in PBDE-209-Exposed Mice Brain
Joint Authors
Verma, Priya
Gupta, Rajaneesh K.
Gandhi, Behrose S.
Singh, Poonam
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
CDRI-08 is a standardized bacoside enriched ethanolic extract of Bacopa monnieri, a nootropic plant.
We reported that CDRI-08 attenuated oxidative stress and memory impairment in mice, induced by a flame retardant, PBDE-209.
In order to explore the mechanism, present study was designed to examine the role of CDRI-08 on the expression of NMDAR1 (NR1) and the binding of REST/NRSF to NR1 promoter against postnatal exposure of PBDE-209.
Male mice pups were orally supplemented with CDRI-08 at the doses of 40, 80, or 120 mg/kg along with PBDE-209 (20 mg/kg) during PND 3–10 and frontal cortex and hippocampus were collected at PND 11 and 60 to study the expression and regulation of NR1 by RT-PCR and electrophoretic mobility shift assay, respectively.
The findings showed upregulated expression of NR1 and decreased binding of REST/NRSF to NR1 promoter after postnatal exposure of PBDE-209.
Interestingly, supplementation with CDRI-08 significantly restored the expression of NR1 and binding of REST/NRSF to NR1 promoter near to the control value at the dose of 120 mg/kg.
In conclusion, the results suggest that CDRI-08 possibly acts on glutamatergic system through expression and regulation of NR1 and may restore memory, impaired by PBDE-209 as reported in our previous study.
American Psychological Association (APA)
Verma, Priya& Gupta, Rajaneesh K.& Gandhi, Behrose S.& Singh, Poonam. 2015. CDRI-08 Attenuates RESTNRSF-Mediated Expression of NMDAR1 Gene in PBDE-209-Exposed Mice Brain. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061464
Modern Language Association (MLA)
Verma, Priya…[et al.]. CDRI-08 Attenuates RESTNRSF-Mediated Expression of NMDAR1 Gene in PBDE-209-Exposed Mice Brain. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1061464
American Medical Association (AMA)
Verma, Priya& Gupta, Rajaneesh K.& Gandhi, Behrose S.& Singh, Poonam. CDRI-08 Attenuates RESTNRSF-Mediated Expression of NMDAR1 Gene in PBDE-209-Exposed Mice Brain. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061464
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061464